Application: This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum.
Features and Benefits: Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.
Other Notes: Proprietary formulation containing inorganic salts, sodium bicarbonate, essential and non-essential amino acids, vitamins, trace elements, plant hydrolysates, and other organic compounds.
Does not contain antibiotics, antimycotics, L-glutamine, insulin, or transferrin.
Caution: Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Legal Information: EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Storage: 2-8°C
Reconstitution: Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.
Empirical Formula (Hill Notation): C5H8O3