Storage: −20°C
UNSPSC Code: 41105335
RIDADR: NONH for all modes of transport
Application:
DNA Molecular Weight Marker XIV (100 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest or RT-PCR, following separation on agarose gels. The DNA molecular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder) for precise size determination.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
General description:
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 100 to 1500 bp and an additional band of 2642 bp
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Physical form:
Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
Sequence:
The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, and an additional band of 2642 bp. The 500-bp and 1000-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.