Storage: −20°C
UNSPSC Code: 41105335
RIDADR: NONH for all modes of transport
Features and Benefits:
Fragment mixture prepared by cleavage of pUCBM21 DNA with Hpa II, and pUCBM21 DNA with Dra I and Hind III.
Contents
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Application:
DNA Molecular Weight Marker VIII is used for the size determination of DNA in agarose gels by:
• Restriction digests
• PCR
• RT-PCR
Note: Use this marker to size DNA fragments between 19 and 1,114 bp.
This marker is also available in DIG-labeled form (DNA Molecular Weight Marker VIII, DIG-labeled).
General description:
DNA Molecular Weight Marker VIII contains a mixture of 17 DNA fragments.
The product is also available as DNA Molecular Weight Marker VIII, Digoxigenin-labeled.
Size range: 19 to 1114 bp
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Sequence:
The mixture contains 17 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 19, 26, 34/34, 37, 67, 110, 124, 147, 190, 242, 320, 404, 489, 501, 692, 900, and 1114 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.