UNSPSC Code: 41106000
RIDADR: NONH for all modes of transport
Features and Benefits:
The DIG RNA Labeling Mix is especially designed for the use with SP6,T7 and T3 RNA polymerases from Roche which are supplied with an optimized transcription buffer.
RNA labeling with Digoxigenin-11-UTP by in vitro transcription with SP6, T7, and T3 RNA polymerases. DIG-labeled RNA is used in a variety of hybridization techniques:
• Northern blots
• Southern blots
• Dot blots
• Plaque or colony lifts
• RNase protection experiments
• Chromosomes, cells, and tissue sections in situ
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.Â The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
For life science research only. Not for use in diagnostic procedures.
Function tested in the DIG RNA Labeling Kit and in the DIG Nucleic Acid Detection Kit.
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0).