Storage: −20°C
UNSPSC Code: 41106000
RIDADR: NONH for all modes of transport
Features and Benefits:
The DIG RNA Labeling Mix is especially designed for the use with SP6,T7 and T3 RNA polymerases from Roche which are supplied with an optimized transcription buffer.
Application:
RNA labeling with Digoxigenin-11-UTP by in vitro transcription with SP6, T7, and T3 RNA polymerases. DIG-labeled RNA is used in a variety of hybridization techniques:
• Northern blots
• Southern blots
• Dot blots
• Plaque or colony lifts
• RNase protection experiments
• Chromosomes, cells, and tissue sections in situ
General description:
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function tested in the DIG RNA Labeling Kit and in the DIG Nucleic Acid Detection Kit.
Specificity:
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0).