UNSPSC Code: 12352200
Components: Reaction Buffer 5x concentrated; CoCl<sub>2</sub> Solution 25 mM; DIG-ddUTP Solution 1 mM; Recombinant Terminal Transferase 400 U/μl; Control Oligonucleotide, unlabeled 20 pmol/μl; Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl; Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled; Glycogen Solution 20 mg/ml; DNA Dilution Buffer, 50 μg/ml fish sperm DNA
RIDADR: UN 3316 9
Features and Benefits:
• Fast hybridization kinetics, due to the small size of oligonucleotides
• Single-stranded probes, no renaturation during hybridization
• Sequence can be designed according to the experiment
• Specially suited for in situ hybridization; due to their small size, the oligonucleotides readily diffuse into fixed tissues and cells
Application:
For 3′-end labeling of oligonucleotides from 14 to 100 nucleotides in length with DIG-11-ddUTP and recombinant Terminal Transferase. DIG-labeled oligonucleotides are used in a variety of hybridization techniques:
• Dot/slot blots
• Colony/ plaque hybridizations
• Southern blots/ Northern blots
• In situ hybridizations
General description:
Kit for 3′-end labeling of oligonucleotides. In this method, the enzyme terminal transferase catalyzes the addition of single digoxigenin-labeled dideoxyuridine triphosphates to the 3′-OH end of oligonucleotides to be labeled.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function tested in a dot blot.
Preparation Note:
Activator: sodium sulfate, Tris
Working concentration: Oligonucleotides: 100 pmol
Up to 100 pmol (1 μg of a 30-mer) oligonucleotide can be labeled in a single standard labeling reaction.
Principle:
One DIG-ddUTP molecule is added to the 3′-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction.