Synonyms: dna labeling and detection kit, dig
Storage: −20°C
UNSPSC Code: 41106000
Components: Unlabeled Control DNA 1 100 µg/ml; Unlabeled Control DNA 2 200 µg/ml; DNA Dilution Buffer; DIG-labeled Control DNA 5.2 µg/ml; Hexanucleotide Mix 10x concentrated; dNTP Labeling Mixture 10x concentrated; Klenow Enzyme, Labeling grade 2 U/µl; Anti-digoxigenin-AP-conjugate antibody 750 U/ml; NBT/BCIP Concentrated Stock Solution; Blocking Reagent
RIDADR: NONH for all modes of transport
Application:
For random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile and color detection of DIG-labeled hybrids. DIG-labeled probes are used in a variety of hybridization techniques:
• Southern blots
• Northern blots
• Dot blots
• Colony and plaque screening
DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light blue precipitate.
General description:
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working concentration: Dilution of Antibody 1:5,000
Specificity:
Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.