A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
Synonyms: DAF-FM DA - CAS 254109-22-3 - Calbiochem; 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
Molecular Formula: C25H18F2N2O7
Molecular Weight: 496.42
Linear Structural Formula: C25H18F2N2O7
MDL Number: MFCD16872050
Purity: >=98% (HPLC)
Biochem Physiol Actions: Primary TargetNitric oxide (NO) fluorescent indicator
Legal Information: Manufactured by Daiichi Pure Chemicals Co., Ltd
Other Notes: Kojima, H., et al. 2001. J. Neurochem.76, 1404.Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.Itoh, Y., et al. 2000. Anal. Biochem.287, 203.Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.