A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.
Synonyms: DAF-2 - CAS 205391-01-1 - Calbiochem; 4,5-Diaminofluorescein
Molecular Formula: C20H14N2O5
Molecular Weight: 362.34
Linear Structural Formula: C20H14N2O5
MDL Number: MFCD05865100
Purity: >=98% (HPLC)
Biochem Physiol Actions: Primary TargetFluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Legal Information: Manufactured by Daiichi Pure Chemicals Co., Ltd
Other Notes: Berkels, R., et al. 2000. Cell Calcium27, 281.Kojima, H., et al. 1998. Anal. Chem. 70, 2446.Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.