Storage: -196C
UNSPSC Code: 12352200
Cell Line Description: Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice. This CACO-2 catalogue number has undergone testing for intestinal permeability: Dome, microvilli and tight junction formation and transport functionality tested and confirmed present at ECACC.
Cell Line Origin: Human caucasian colon adenocarcinoma, intestinal permeability characteristics tested
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.
DNA Profile: STR-PCR Data: Amelogenin: XCSF1PO: 11 D13S317: 11,13,14D16S539: 12,13D5S818: 12,13D7S820: 11,12THO1: 6TPOX: 9,11vWA: 16,18
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm-¦ using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.
Other Notes: NaviCyte Scientific holds the exclusive commercial distribution rights to the CACO-2 cell line deposited by the Memorial Sloan-Kettering Cancer Center. Note: All uses of Catalogue Numbers 86010202 and 09042001, other than for research by a non-commercial or academic entity, require a license and use authorization from NaviCyte Scientific under its exclusive arrangement with Memorial Sloan-Kettering Cancer Center. For information on the licensing terms, please contact NaviCyte Scientific via contact@navicyte-scientific.com or (+1) 973-868-6100.