Synonyms: Anti-EIF-2B; Anti-EIF2B-Beta; Anti-EIF2BB; Anti-Eukaryotic translation initiation factor 2B, Beta; Anti-Eukaryotic translation initiation factor 2B, subunit 2
UNSPSC Code: 12352203
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Application: Anti-eIF2Bbeta (N-terminal) antibody produced in rabbit is suitable for the following applications: . Immunoprecipitation at a concentration of 3-5mug using K562 cell lysates. Indirect immunofluorescence (at a concentration of 2-5mug/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human eIF2Bbeta). Western blotting at a concentration of 2-4mug/mL using K562 or AT3B1 cell lysatesYale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if
Biochem/physiol Actions: Translation initiation factor eIF-2B subunit Î² is a protein encoded by EIF2B2 gene in humans. It is a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. It is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders.
Immunogen: synthetic peptide corresponding to amino acids 7-24 of human eIF2Bbeta, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in mouse.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport