Synonyms: Anti-DCP2 decapping enzyme homolog (S. cerevisiae); Anti-NUDT20; Anti-Nucleleoside diphosphate-linked moiety X motif 20; Anti-Nudix motif 20
UNSPSC Code: 12352203
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General description: Dcp2 colocalizes with Dcp1 in distinct cytoplasmic foci along with other proteins involved in the 5' to 3' mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. hDCP2 contains a highly conserved Nudix (nucleoside diphosphate linked to an X moiety) motif critical for the decapping activity.
Application: Anti-DCP2 antibody produced in rabbit is suitable for indirect immunofluorescence at a working concentration of 2-5mug/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP2 and western blot analysis at a working concentration of 2-4mug/mL using lysates of K-562 and Rat1 cells. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Biochem/physiol Actions: Dcp2 is an RNA binding protein and can cleave only a cap structure that is linked to an RNA moiety, suggesting that Dcp2 can differentially associate with specific mRNAs. Dcp2 cleaves the m7G mRNA cap in the 5' to 3' mRNA decay pathway, in association with Dcp1 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5' mRNA. Dcp2 is the catalytic subunit, and the mRNA is degraded by the major cytoplasmic 5â€² to 3â€² exonuclease XRN1. The enzymatic activity of DCP2 is critically dependent on the DCP1 subunit in vivo.
Immunogen: synthetic peptide corresponding to amino acids 406-420 of human DCP2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Target description: DCP2 (C-terminal) is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end.
RIDADR: NONH for all modes of transport