Synonyms: (S)-2-{4-[(2,4-Diaminopteridin-6-yl)methylamino]benzamido}pentanedioic acid; 4-Amino-PGA; 4-Aminofolic acid; 4-Aminopteroyl-L-glutamic acid
CAS Number: 54-62-6
Formula: C19H20N8O5
Formula Weight: 440.41
Linear Formula: C19H20N8O5
Storage: -20C
Beilstein Registry Number: 69045
EC Number: 200-209-9
UNSPSC Code: 12352200
Application: Aminopterin concentrate supplement for hybridoma cell culture applications.
Biochem/physiol Actions: Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by gamma-glutamyl hydrolase.
Linkage: More potent, but more toxic, than methotrexate.
Reconstitution: Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 0.4 muM aminopterin.
Other Notes: Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. A5159.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.
RTECS: MA1050000
RIDADR: UN 2811 6.1 / PGI
WGK Germany: 3
R Codes: 61-28
S Codes: 53-28-36/37-45
IOD Codes: T+
Symbol: GHS06, GHS08
Signal Word: Danger
Hazard Statements: H300-H360
Precautionary statements: P201-P264-P301 + P310-P308 + P313