Synonym(s): BSA; Bovine albumin
CAS No.: 9048-46-8
Purity: ≥96% (agarose gel electrophoresis)
Application: This albumin has been qualified for use in media designed to support in vitro applications such as mouse blastocyst and morulae culture.
Preparation Note: Serum albumin may be referred to as Cohn Fraction V. This naming convention is taken from the original Cohn1 method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Cohn Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage: 2-8°C
EC No.: 232-936-2
Biochem/physiol Actions: Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Empirical Formula (Hill Notation): C8H10O2