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GenScript

MonoRab™ Anti-Camelid VHH Affinity Resin

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MonoRab™ Anti-Camelid VHH Affinity Resin

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Camelid VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx) is a peptide chain about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. This single-domain antibody has a broad range of applications in biotechnical and therapeutic research fields due to its small size, simple production and high affinity.

GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified Camelid VHH single domain antibody is eluted with a high recovery rate. Table 1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin.

For laboratory or further manufacturing use only.

Product Content50% settled resin in TBS with 0.02% sodium azide
Matrix4% cross-linked agarose
Average Bead Size90 μm
LigandMonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb
Binding CapacityApproximately 2-4 mg Camelid VHH  protein per mL settled resin (depending on amino acid sequence of different Camelid VHHs)
Storage and StabilityStore at 2-8 °C for up to 12 months. Do not freeze the resin
Resin ReuseWhen stored at 2 - 8° C, the resin can be recycled at least 5 times with no loss of binding capability. The resin can be reused up to 10 times with minimum loss of binding capability.
Elution MethodAcid buffer elution (pH 2.5)
Reagents CompatibilityCompatible with commonly used bacterial lysis reagents. For reagents compatible with purification, see tables 2 and 3 in the manual.

 

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Product Detail
Thomas No.
CHM03U899
Mfr. No.
L00905-1
Description
MonoRab™ Anti-Camelid VHH Affinity Resin, 1mL
list price/quantitytotal
$0.00
Thomas No.
CHM03U900
Mfr. No.
L00905-5
Description
MonoRab™ Anti-Camelid VHH Affinity Resin, 5mL
list price/quantitytotal
$0.00
Thomas No.
CHM03U901
Mfr. No.
L00905-10
Description
MonoRab™ Anti-Camelid VHH Affinity Resin, 10mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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