Iron-responsive element-binding protein 2 (UniProt: P48200; also known as IRE-BP 2, Iron regulatory protein 2, IRP2) is encoded by the IREB2 gene (Gene ID: 3658) in human. IRP2 is a member of the aconitase/IPM isomerase family that serves as an RNA-binding protein, which regulates the homeostatic binding of intracellular iron to its specific cognate mRNA hairpin structures known as iron-responsive elements (IREs). Its interaction with IRE is shown to be abolished under conditions of high iron. In iron rich cells and in the presence of oxygen, IRP2 is ubiquitinated and degraded in proteasome pathway. Its ubiquitination is achieved by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. IN IRP2, Cys201 and His204 have been shown to be critical for IRP2 degradation. Cys201 binds ferric heme and His204 binds ferrous heme and they are involved in sensing the redox state of the heme iron and in generation the oxidative modification. Two isoforms of IRP2 have been described that are produced by alternative splicing. (Ref.: Ashizuka, M., et al. (2002). Mol. Cell. Biol. 22(18); 6375-6383; Ishikawa, H., et al. (2005). Mol. Cell 19(2); 171-181).
Synonyms: Iron-responsive element-binding protein 2, IRE-BP 2, Iron regulatory protein 2
Application: Research CategorySignaling
Other Notes: Concentration: Please refer to lot specific datasheet.