Synonyms: Anti-BAT8; Anti-G9a/NG36; Anti-NG36
MDL No.: MFCD06411814
Storage: -20C
UNSPSC Code: 12352203
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Application: Anti-G9a Methyltransferase antibody produced in rabbit is suitable for use in western blotting of . nuclear lysates from HeLa cells. proteins extracted from HCT116 and 293T cells in a study to show that G9a is not essential for maintenance of DNA methylation in somatic cellsIt is suitable for immunoprecipitation at a working concentration of 1-2μg using 293-T cell extracts and microarray. It is also suitable for western blot analysis ata working concentration of 1-2μg/mL using nuclear extracts of 293-T cells.
Biochem/physiol Actions: G9a is a lysine-preferring histone methyltransferase (HMTase) containing a SET domain. It has 10 to 20-fold stronger in vitro HMTase activity towards histone H3-Lys9 (H3-K9) when compared to Suv39H1, another member of the same family of HMTases. It also methylates Lys27 of histone H3. Suv39 h1 methylates only lysine 9. It is capable of mono and dimethylation within silent euchromatin, whereas Suv39h1 trimethylates at pericentric heterochromatin. G9a is an important factor in the early embryonic development and plays a dominant role in H3-K9 methylation of euchromatin in vivo. It also forms a crucial component of the CtBP and E2F-6 transcriptional repressor complexes.
Immunogen: synthetic peptide corresponding to amino acids 1193-1210 of human G9a, conjugated to KLH via an N-terminal added lysine residue. This sequence differs from the mouse sequence by 3 amino acids.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Specificity: The antibody recognizes the two isoforms of G9a methyltransferase (~180 kDa and 160 kDa).
RIDADR: NONH for all modes of transport
WGK Germany: nwg