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Jackson Immunoresearch

AffiniPure Goat Anti-Rabbit IgG (H+L)

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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Target: Rabbit

Host: Goat

Antibody Format: Whole IgG

Specificity: IgG (H+L)

Conjugate: Unconjugated

Clonality: Polyclonal

Physical StateFreeze-dried powder
StorageStore at 2-8°C under sterile conditions. Prepare working dilution fresh each day.
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6  
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
PreservativeNone
Suggested Working Concentration or Dilution Range
ELISA10-20 µg / ml
Western Blot10-20 µg / ml
Histo-/Cyto-Chemistry:10-20 µg / ml
Flow Cytometry10-20 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840M44
Mfr. No.
111-005-003
Description
AffiniPure Goat Anti-Rabbit IgG (H+L), 2 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C840M44 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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