Protamine Sulfate from Salmon Sperm

Protamine sulfate is a purified mixture of simple protein principles obtained from the sperm or testes of suitable species of fish, which has the property of neutralizing heparin. Because of having much basic amino acids (mostly arginine) protamine contains far greater nitrogen than other proteins. Its molecular weight is relatively small. Histone and other basic proteins in the testes of unmatured fishes convert into protamine along with the growth of the fishes. In the testes protamine takes the form of nucleoprotamine linked with DNA.

Protamine Sulfate is a raw materials for lab to study preparations like insulin compounds, etc. It is used in separation and refining of vaccines. It is a reagent for removing nucleic acids from enzyme solution for the purpose of easy separation and refining. Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Inhibits lipoprotein lipase. Protamine sulfate shown to inhibit the classical pathway of complement. It inhibits turnover of lipoproteins by lipoprotein lipase.

USP/NF Activity: Each mg of protamine sulfate, calculated on the dried basis, neutralizes not less than 80 USP units of heparin activity derived from lung tissue or not less than 100 USP units of heparin activity derived from intestinal mucosa.