The product is in 10mM phosphate, 0.15M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol containing 0.1% proclin as preservative.
Protein A is a component of the cell wall of Staphylococcus aureus (Cowan 1 Strain), and binds to the Fc portion of immunoglobulin molecules of most mammalian species. It is composed of a single polypeptide chain of molecular weight 42,000. Protein A binds IgG molecules and, in some cases, IgM and IgA.
Peroxidase-Conjugated Protein A is a versatile reagent which is useful in many immunological assay systems. It is intended for use in cell and tissue staining, dotting and blotting, ELISA and other immunoassays. For each specific experiment, the product should be titered to maximize signal to background.
Product is a conjugate of Protein A and highly purified (Rz >3.0) horseradish peroxidase.
Key Applications: Dot Blot, ELISA, Western Blotting, Immunohistochemistry
Application Areas: Immunoassays
Product Type: Antibodies
Antibody Type: Protein A & G
Conjugate: HRP
Species Reactivity: Binding Characteristics of Protein A: Human, Rabbit, Mouse, Sheep, Goat, Rat, Dog, Guinea pig; Pig; Cow
Format: Liquid
NOTES: Technique: Cell/tissue staining; Dotting/blotting 1:500 - 1:1,000; Enzyme Immunoassays Substrate solution = 1 mM ABTS in 0.1M citrate buffer, pH 4.2, containing 0.03% hydrogen peroxide. ELISA Titer- 1:110,000
Storage & Handling: Store unopened product at 2-8°C. Do not freeze. If accidentally frozen, centrifuge to clarify and precipitate before use.