nterleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 has multiple effects on both hematopoietic and non-hematopoietic cells. Many of the biological effects described for IL-11 overlap with those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell-dependent development of specific immunoglobulin-secreting B cells.
Recombinant Human IL-11 Fc Chimera produced in CHO cells is a polypeptide chain with the C-terminal human IgG1 Fc fragment. A fully biologically active molecule, rhIL-11 a molecular mass of 51.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Synonyms AGIF; IL-11; Interleukin-11; IL11; Interleukin11; AGIFoprelvekin; IL-11Oprelvekin
Description Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 has multiple effects on both hematopoietic and non-hematopoietic cells. Many of the biological effects described for IL-11 overlap with those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell-dependent development of specific immunoglobulin-secreting B cells. Recombinant Human IL-11 Fc Chimera produced in CHO cells is a polypeptide chain with the C-terminal human IgG1 Fc fragment. A fully biologically active molecule, rhIL-11 a molecular mass of 51.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Species Human
Source CHO
Tag C-terminal human IgG1 Fc Chimera.
Biological Activity In testing.
Sequence Accession#P20809.1 (Pro22-Leu199)
Properties
- Molecular Weight 51.7 kDa, observed by reducing SDS-PAGE. Purity > 85% as analyzed by reducing SDS-PAGE
- Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
- Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
- Endotoxin Level < 1 EU/μg, determined by LAL method.
- Storage Lyophilized recombinant IL-11 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, IL-11 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
- Note For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.