Animal-free Recombinant Human Noggin is expressed in an engineered human 293 cell expression system with serum- free, chemically defined media. The protein is a highly stable, authentically glycosylated, disulfide linked dimer. Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS-PAGE analysis under reducing conditions.