Avidin is a basic glycoprotein isolated from raw egg white. It exhibits high binding affinity for biotin and is capable of producing biotin deficiency in rats and chicks. It also occurs in the white portion of eggs and the oviducts of birds and amphibia. It is destroyed by cooking or irradiation.
Avidin is useful in a biotin-avidin system for labeling biomolecules for receptor studies, immunoassays and immunohistological methods. Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol. Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies. The product has also been used as a blocking agent in ELISA applications.
One unit will bind 1 ug of D-biotin at pH 8.9.
Specific Activity: 10-15 u/mg solid
Key Applications: Labeling biomolecules for receptor studies, immunoassays and immunohistological methods
Application Areas: Molecular biology
Product Type: Proteins, Enzymes & Peptides
Presentation: White Crystalline Powder
Format: Crystalline Powder
Solubility: Soluble in water or dilute aqueous buffer.
Storage & Handling: Store at -20°C.