Synonyms: Yeast Extract Peptone Dextrose Broth
YPD is a nutritionally rich medium for the growth of S. cerevisiae, containing dextrose, peptone and yeast extract. Dextrose is the energy source. Yeast extract and peptone provide carbon, nitrogen, minerals, vitamins, trace ingredients and other essential growth nutrients that stimulate the growth of yeasts.
Applications
YPD Broth is used for routine growth of Saccharomyces cerevisiae for molecular biology studies.
Preparation Method
Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in YPD Broth formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121°C for 15 minutes.
Recommended Use
Add 10 large capsules per liter of purified water.
Typical Working Concentration
50 g/L