Generally, goat anti-human or donkey anti-human IgG polyclonal antibodies are used as the secondary antibody to detect human primary antibodies. However, since polyclonal antibodies can recognize many epitopes, the background and the cross reactivity in the assays is usually high, making the results hard to interpret.
To minimize the background and cross reactivity and improve the lot-to-lot consistency, a unique GenScript Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb has been developed to be used as a secondary antibody. The superiority of this antibody is that it can be used as an idiotype antibody to detect drug metabolism in vivo.