Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. Proinsulin is the prohormone precursor to insulin made in pancreas. It is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids; 4 are removed altogether, and the remaining 31 form the C-Peptide. The C-Peptide is abstracted from the center of the proinsulin sequence; the two other ends (α and β chains) remain connected by disulfide bonds. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
GenScript Insulin Antibody (6E9F1), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant Insulin expressed in yeast.