Sharing 41% sequence identity with human Interferon gamma (hIFN--?), mouse IFN gamma (mIFN--?)is a macrophage-activating factor.The active form of IFN--? is an antiparallel dimer that sets off IFN--?/JAK/STAT pathway. IFN--? signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--?–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--? is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers.
Recombinant mouse IFN gamma (rmIFN-?) produced in E. coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--? has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.