Green Taq DNA Polymerase is designed to increase the stability of the Taq enzyme for more convenient transport and applications. The polymerase (1000 U) is designed for 400 rxns if 2.5 U are used per 50 ul PCR reaction. It can be stable for six months if stored at 4°C or for up to one month without significant loss of activity at ambient temperature. Our technology can increase the stability of Green Taq DNA at higher temperature (72°C). The higher yield PCR product can be harvested in long PCR amplification.
Formulation
PCR Reaction Buffer (with Mg 2+ ): 100 mM Tris-HCl, 15 mM MgCl 2 , 500 mM KCl and 1% Triton X-100, pH 8.3 (4°C).
Enzyme Storage Buffer: 20 mM Tris-HCl, 1 mM DTT,0.1 mM EDTA, 0.1 M KCl, 0.5% Nonidet P40 (v/v), 0.5% Tween 20 (v/v), 50% glycerol(v/v), pH 8.0 (4°C)
Applications
The applications of Green Taq DNA Polymerase include the following:
- PCR(For simple templates, this enzyme can be optimized for amplification of PCR products up to 10 kb; However, for complex templates, this enzyme can be used for amplification of PCR products up to 3 kb.)
- 3'A-tailing of blunt ends (T/A-cloning)
- Primer extension
- DNA labeling reactions