ZymoPURE™ 96 Plasmid Miniprep Kit The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The unique vacuum plate design provides zero buffer retention and low elution volumes. In addition, the provided wash plate also significantly reduces plasmid DNA cross-contamination between wells relative to other popular high-throughput plasmid prep kits. ZymoPURE™ technology uses a modified alkaline lysis method and our patented binding chemistry, which enables the highest DNA binding capacity and rapid loading of the lysate and wash buffer, resulting in the purification of highly concentrated (up to 1 µg/µl) plasmid DNA directly from a 96-well vacuum plate. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR and other sensitive downstream applications. As an added convenience, the ZymoPURE™ 96 Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. ZymoPURE™ Filter Plates are also included for rapid clearing of the lysate and the unique Zymo-Spin P-96 Plate design allows the binding step to be performed using a vacuum or centrifuge. EZ-Vac 96 Vacuum Manifold The EZ-Vac 96 Vacuum Manifold is designed for the high-throughput, rapid parallel manual purification of nucleic acid using the ZymoPURE 96 Plasmid Miniprep Kit. The vacuum manifold allows researchers to simplify their nucleic acid purification workflows further by eliminating the need for multiple centrifugation steps through the use of a vacuum. The unique manifold design also conveniently allows the filtrate and wash solutions to be collected in an external catch flask (not included), eliminating the need for manifold disassembly and waste handling. ZymoPURE Filter Plate/Wash Plate Available as a refill for the ZymoPURE 96 Plasmid Miniprep Kit. The ZymoPURE Filter Plate/Wash Plate can be used with centrifuges and vacuum manifolds for the rapid clarification of bacterial lysates when used in conjunction with our ZymoPURE Plasmid Prep buffers. The capacity of the well is 1.0/1.1/1.3 mL