The SPINeasy® DNA/RNA Kit for Soil integrates our proprietary inhibitor removal and spin column technology for selective binding of DNA (blue column) and RNA (white column) from a single soil sample. Humic acids, heavy metals, and other aromatic components are among the most notorious PCR inhibitors found in soil samples, leading to false negative or inaccurate results. Non-optimized nucleic acid extraction protocols often co-purify inhibitors.
The SPINeasy DNA/RNA Kit for Soil allows for fast and simultaneous isolation of pure microbial genomic DNA and RNA from challenging soil samples, including those with low biomass or those that are highly contaminated. The extracted nucleic acids lack contaminants and are suitable for use in downstream applications, such as qPCR, RT-qPCR, and sequencing, without the need for further inhibitor removal steps.
Application Notes:
The SPINeasy® DNA/RNA Kit for Soil integrates our proprietary inhibitor removal expertise and our new technology for selective binding of DNA and RNA. The SPINeasy® DNA/RNA Kit for Soil allows simultaneous isolation of pure microbiome genomic DNA and RNA from challenging soil types, including those with low biomass or those that are highly contaminated. The isolated nucleic acid products showed no contaminants and were immediately ready for use in downstream applications, including qPCR and RT-qPCR without the need of further inhibitor removal step.
Key Applications:
Genomic, Molecular Biology, PCR, qPCR
Key features and details
- Simultaneous isolation of microbial DNA and RNA from various soil samples
- Inhibitor removal technology extracts nucleic acids free of most notorious PCR inhibitors
- Lysing Matrix YB tubes provide efficient and reproducible sample lysis with FastPrep instruments
- High yield and quality DNA/RNA from challenging soil samples, including high and low biomass or highly contaminated samples
Application Notes: The SPINeasy® DNA/RNA Kit for Soil integrates our proprietary inhibitor removal expertise and our new technology for selective binding of DNA and RNA. The SPINeasy® DNA/RNA Kit for Soil allows simultaneous isolation of pure microbiome genomic DNA and RNA from challenging soil types, including those with low biomass or those that are highly contaminated. The isolated nucleic acid products showed no contaminants and were immediately ready for use in downstream applications, including qPCR and RT-qPCR without the need of further inhibitor removal step.
Base Catalog Number: 6554050
Brand: SPINeasy
Components: Equilibration buffer (24 mL), Lysing Matrix YB (50 ea), Buffer SDR1A (18 mL), Buffer SDR1B (36 mL), Buffer SDR2 (24 mL), Buffer SDR3 (45 mL), Buffer SDR4 (45 mL), Buffer SDR5 (90 mL), Buffer SDR6 (150 mL), RNase Free Water (15 mL), DNA Column (50 ea), RNA Column (50 ea), 2 mL Microcentrifuge Tubes (100 ea), DNA Collection Tubes (250 ea), RNA Collection Tubes (150 ea), DNA Elution Tubes (50 ea), RNA Elution Tubes (50 ea)
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Storage: Store at Room Temperature (15-25?), Dry environment