The SPINeasy DNA Pro Kit for Soil has been carefully designed for the isolation of pure microbiome genomic DNA from challenging soil types, including those with low biomass or that are highly contaminated. Soil samples are complex environments characterized by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components, which often prove to be challenging for downstream molecular analysis.
The SPINeasy DNA Pro Kit for Soil effectively lyses microbial populations, including bacteria, fungi, viruses, and protists. The kit provides similar yields to that of our highly cited FastDNA™ SPIN Kit, but with improved purity and reduced processing time. Isolated DNA products showed no inhibition in PCR and were immediately ready to be used for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Application Notes:
The SPINeasy DNA Pro Kit for Soil quickly and effectively isolates microbial gDNA from various soil samples. This kit is ideal for soil microbiome studies from low and high biomass soils, as it provides unbiased lysis of various microbial populations, such as bacteria, fungi, viruses, and protists. Isolated DNA exhibited a lack of inhibition in PCR and was immediately ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Usage Statement:
Research Use Only (RUO)
Key Applications:
Genomic, Molecular Biology, Nucleic Acid Isolation & Purification, PCR, qPCR, NGS, Shotgun sequencing, Microbiome analysis
Key features and details
- Isolation of microbial DNA from high and low biomass soil samples
- Unbiased lysis of microbial populations for more accurate microbiome studies
- Inhibitor removal technology extracts nucleic acids free of most notorious PCR inhibitors
- Lysing Matrix YB tubes provide efficient and reproducible sample lysis with FastPrep instruments
- Purified nucleic acids are ready for downstream applications such as PCR, qPCR, 16S/ITS, and shotgun sequencing
Alternate Names: SPINeasy Pro Soil Kit, DNA Extraction Kit for Soil
Application Notes: The SPINeasy DNA Pro Kit for Soil quickly and effectively isolates microbial gDNA from various soil samples. This kit is ideal for soil microbiome studies from low and high biomass soils, as it provides unbiased lysis of various microbial populations, such as bacteria, fungi, viruses, and protists. Isolated DNA exhibited a lack of inhibition in PCR and was immediately ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Base Catalog Number: 65460
Brand: SPINeasy
Components: 50x Lysing Matrix YB, 2 mL RNase A Solution, 55 mL Buffer S1, 22 mL Buffer S2, 50 mL Buffer S3, 28 mL Buffer S4, 40 mL Buffer S5, 6 mL Buffer S6, 50x Column S, 50x 2 mL collection tubes, 50x 1.5 mL collection tubes
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Sample Type: Up to 500 mg of soil samples, including sand, alkaline soil, clay, sediment, and topsoil
Storage: Store at Room Temperature (15-25?) in a dry environment
Usage Statement: Research Use Only (RUO).