The SPINeasy DNA Pro Kit for Feces - efficiently isolates high-quality gDNA from stool samples. Fecal samples present distinct sample processing challenges due to sample composition which is largely dependent on diet and may include fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. These compounds may impair sample homogenization, decreasing both the quantity and quality of the extracted DNA.
The SPINeasy DNA Pro Kit for Feces was designed to address these challenges. Samples are optimally homogenized by bead beating with new Lysing Matrix YB tubes, Lysis Buffer SF1, and FastPrep instruments. Contaminants, including humic acids and RNA, are efficiently removed by treatment with Buffer SF2, while Buffer SF3 enables the specific binding of DNA to spin columns, without the need for RNase treatment. The final DNA product is ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need for further inhibitor removal steps.
Application Notes:
The SPINeasy DNA Pro Kit for Feces allows for rapid isolation of high-quality, inhibitor-free genomic DNA from fecal samples. The kit effectively addresses common extraction challenges present with this sample type. Lysing Matrix tubes and specially designed lysis buffers remove common inhibitors and contaminants that co-purify with DNA. The final DNA product is ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing, without the need for further inhibitor removal steps and RNase treatment.
Usage Statement:
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.
Key Applications:
Molecular Biology, Nucleic Acid Isolation and purification, PCR, qPCR, Gut microbiome analysis, NGS, shotgun sequencing
Key features and details
- Efficient and rapid isolation of high-quality genomic DNA from both fecal and soil samples
- Optimized Buffer SF3 enables specific binding of DNA without RNA contamination
- New Lysing Matrix YB tubes and Lysis Buffers provide efficient and reproducible sample lysis with FastPrep instruments?
- Extracted DNA is free of PCR inhibitors and ready for downstream applications, such as PCR, qPCR, 16S/ITS, and shotgun sequencing
Alternate Names: SPINeasy DNA Kit for Feces, DNA Extraction Kit for Feces, Spin Column DNA Extraction and Purification Kit for Feces
Application Notes: The SPINeasy DNA Pro Kit for Feces allows for rapid isolation of high-quality, inhibitor-free genomic DNA from fecal samples. The kit effectively addresses common extraction challenges present with this sample type. Lysing Matrix tubes and specially designed lysis buffers remove common inhibitors and contaminants that co-purify with DNA. The final DNA product is ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing, without the need for further inhibitor removal steps and RNase treatment.
Base Catalog Number: 6547050
Brand: SPINeasy
Components: 50x Lysing Matrix YB, 45 mL Buffer SF1, 12 mL Buffer SF2, 35 mL Buffer SF3, 40 mL Buffer SF4, 40 mL Buffer SF5, 7.5 mL Buffer SF6, 50x Column SF with collection tubes
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Sample Type: Up to 250 mg per prep of fecal/stool samples
Storage: Store at Room Temperature (15-25°C) in a dry environment.
Usage Statement: Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.