SPINeasy DNA Kit for Water is a high-performance water genomic DNA extraction kit based on silica membrane spin column technology. The kit has been specifically designed to concentrate microbes present in water samples by filtration and transfer to a Lysing Matrix tube (4.5 mL) for the best extraction of microbial gDNA from water in less than 30 min. Water samples are processed using our uniquely formulated Lysis Buffer W1 and Lysing Matrix E to effectively lyse various types of microbial cells, including bacteria, fungi, virus, and protozoans. Column W1 (provided with the kit) has high binding capacity and selectivity for gDNA. The combination of components in the kit helps extract microbial gDNA of high yield and purity that is ready for downstream applications, such as restriction digestion, PCR, and NGS.
Application Notes:
Extract gDNA of high yield and purity from different types of water including river water, pond water, seawater, sewage that is ready for downstream analyses such as PCR, restriction digestion and sequencing.
Usage Statement:
Research Use Only (RUO)
Key Applications:
Genomic research; Environmental study
Key features and details
- Specifically designed for the isolation of microbial DNA from various water samples
- Fast and easy extraction of gDNA in less than 30 min
- Supplied with filter membranes and 4.5 mL Lysing matrix tubes to capture and concentrate microbes and efficiently homogenize in using a FastPrep instrument
Alternate Names: SPINeasy DNA Extraction Kit for Water; gDNA Extraction Kit for Water
Application Notes: Extract gDNA of high yield and purity from different types of water including river water, pond water, seawater, sewage that is ready for downstream analyses such as PCR, restriction digestion and sequencing.
Base Catalog Number: 6536050
Brand: SPINeasy
Components: 50 x 5 mL Lysing Matrix E, 60 mL Lysis Buffer W1, 8 mL Lysis Buffer W2, 550 μL RNase A Solution, 15 mL Inhibitor Removal W, 30 mL Binding Buffer W, 9 mL Wash Buffer W1, 6 mL Wash Buffer W2, 10 mL DES Buffer, 50 x Filter Membrane, 50 x Column W1, 50 x 2.0 mL Collection Tubes, 50 x 1.5 mL Collection Tubes
Hazard Statements: Hazard Class 8
Pack Size: 50 preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Sample Type: Water samples including fresh and sea water, pond and river water, wastewater, etc.
Storage: Store at Room Temperature (15-30 °C), Dry environment.
Usage Statement: Research Use Only (RUO).