Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure.
Triton X-100 is a non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered as a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It does absorb in the ultraviolet region of the spectrum, however, so can interfere with protein quantitation.
The principal uses of Triton® X-100 is in industrial and household detergent applications and in emulsifying agents. It is employed in almost every type of liquid, paste, and powdered cleaning compound, ranging from heavy-duty industrial products to gentle detergents for fine fabrics. It is also important ingredient of primary emulsifier mixtures used in the manufacture of emulsion polymers, stabilizers in latex polymers, and emulsifiers for agricultural emulsion concentrates, and wettable powders.
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Grade: Molecular biology reagent
Key Applications: Surfactant, Immunostaining, Decellularization of animal-derived tissues, Detergent
Product Type: Biochemicals
Biochemical Category: Surfactants
Density: 8.9 lb/gal(Lit.)
Specific Gravity: 25 °/25°C=1.065 g/mL(Lit.)
Viscosity: 240 Brookfield, at 25°C, cP(Lit.)
Presentation: Clear, Colorless to Yellow, Viscous Liquid
Format: Viscous Liquid
Color (APHA): ≤100
pH: 5- 8(5% aq soln)
Aggregation Number: 140(Lit.)
Critical Micelle Concentration (CMC): 0.24 mM(lit.)
Surface Area: 48-54 per molecule in square Angstroms(Lit.)
NOTES: Cloud point=63 - 69°C(1% aq soln); Pour Point °F=45; HLB Value=13.5; Average EO Units=9-10
Solubility: Miscible in Water, Polar Organic Solvents (alcohols, glycols, ethers, ketones, etc.) & Aromatic Hydrocarbons; Soluble in Inorganic Salt Solutions & Aqueous Mineral Acids; Insoluble in Aliphatic Hydrocarbons.
Storage & Handling: Store at Room Temperature(15-30°C)