SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on protein size. The salt is of an organosulfate consisting of a 12-carbon tail attached to a sulfate group, giving the material the amphiphilic properties required of a detergent.
SDS is an detergent typically used to solubilize and denature proteins for electrophoresis. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. SDS is used in Protein Electrophoresis & Blotting.
Key Applications: Denature proteins, Surfactant, Electrophoresis, Chromatography, Detergents
Application Areas: Protein Electrophoresis & Blotting
Research Areas: Cell biology
Product Type: Biochemicals
Biochemical Category: Surfactants
Chemical Class: Surfactants
Density: > 1.1 at 20°C (water = 1)
Auto Ignition: 248°C (powder containing 90% SLS); 447°C (solution containing 14.8%SLS)
Melting Point: 180 - 210°C
Vapor Pressure: Practically zero (Lit.)
Refractive Index: 1.461(Lit.)
UV/Visible Absorbance: OD260 (1% aq soln) ≤0.1, OD280 (1% aq soln) ≤0.1
Presentation: White Powder
pH: 6 - 7 (1% aq soln)
Aggregation Number: 62
Critical Micelle Concentration (CMC): 8.27 mM
Solubility: Soluble in water (200 mg/mL - clear, faint yellow solution), and ethanol (0.1g/10 mL)
Storage & Handling: Store at Room Temperature (15-30°C).