Polysorbate-20 is a nonionic surfactants derived from sorbitan esters.
Polysorbate 20 is used as an emulsifying agent for the preparation of stable oil-inwater emulsions in pharmaceuticals, cosmetics, and cleaning compounds. Polysorbate 20 has been used in preextraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). It has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. It can be used for lysing mammalian cells at a concentration of 0.05 to 0.5%. It is a nonionic detergent widely used in biochemical applications.
Suitability: complies for IR spectroscopy
Key Applications: Non-ionic detergent
Product Type: Biochemicals
Chemical Class: Surfactants
Density: 1.095 g/mL at 25°C(Lit.)
Specific Gravity: 1.08 - 1.13
Viscosity: Brookfield Viscosity: 370-430 cps (25°C, neat)(Lit.)
Boiling Point: >100°C(Lit.)
Refractive Index: n20/D 1.468(Lit.)
Presentation: Yellow Liquid
Format: Liquid
pH: 5-7 (1% aqueous solution, Lit.)
Critical Micelle Concentration (CMC): 0.06 mM (60 mg/L or 6 × 10-3 mole/L)(Lit.)
Detection Method: FTIR
Water Content: ≤3%
NOTES:
Saponification Number 40 - 50
Solubility: Miscible in Water (1g/20 mL). It is also miscible with alcohol, dioxane, and ethyl acetate; and is practically insoluble in liquid paraffin and fixed oils(such as mineral oil).
Sterilization of Solutions: Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Polysorbate 20 may need to be warmed to about 40°C and alternated with portions of hot distilled water while being poured through the filter.
Storage & Handling: Store at Room Temperature (15-30°C).