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Zymo Research Corporation

ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Rack)

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The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

Applicable For

All sensitive downstream applications such as qPCR and Next-Generation Sequencing.

Elution Volume

≥ 37.5 µl DNA Elution Buffer or DNase free water

Equipment

Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block

Processing Volume

Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells)

Purity

High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing

Sample Source

Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.

Sample Storage

Eluted DNA should be stored at ≤ -20°C.

Size Range

Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.

Type

Total DNA

Yield

Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum)

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Product Detail
Thomas No.
CHM03V974
Mfr. No.
D4302-E
Description
ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Rack)
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