Endotoxin Free pDNA Miniprep Kit is uniquely designed to facilitate ultrapure pDNA free from endotoxins (<0.1EU/μg), ideal for transfection even into highly sensitive mammalian cells. These kits offer high yields from both low copy as well as high copy number plasmid. The innovative buffer system and filter device provides efficient binding of pDNA onto the spin column.
Washing is done with the help of provided wash buffers in order to remove endotoxins, RNA, proteins and polysaccharides. The technology also does away with hasselsome gravitational waiting, phenol extraction (associated with desalting) and ethanol precipitation (associated with anion exchange based purification).
Applications
- Automated Fluorescent Sequencing
- Radioactive Sequencing
- Restriction Digestion
- Transfection (with highly sensitive mammalian cell lines)
- Cloning
- PCR