Synonyms: TAE buffer
Application: TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis.1 Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity.2 Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Other Notes: 0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.
Grade: for molecular biology
Impurities: DNase and RNase, none detected
Product Line: BioReagent
Quality Level: GMP
Sterility: non-sterile; 0.2 μm filtered
Suitability: suitable for gel electrophoresis (after dilution to working concentration)