IBI's RBC Lysis Buffer was designed specifically for DNA isolation from large volumes of blood. However, this highly versatile reagent is able to extract genomic DNA from various other samples, such as cultured cells, tissue, bacteria and fungus. RBC Lysis Buffer is included with the kit to remove non-nucleated red blood cells and to reduce hemoglobin contamination. When the blood sample is less than 50µl or when using nucleated red blood cell samples, the Cultured Cells Protocol is recommended. If a larger sample size is required, the buffer volume can be scaled proportionately.