For quantitative determination of DNA.
- Linear detection range 2 - 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay.
- Convenient. Single working reagent. Can readily automated to test tens of thousands of samples per day.
- Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.
- Method: FL340/450 nm.
- Samples: Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices.
DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling. Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. BioAssay Systems QuantiFluo™ DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at λex/em = 350/450nm, is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.