For quantitative determination of arginase activity and screen for its inhibitors.
- Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format.
- Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent.
- Can be readily automated as a high-throughput assay for thousands of samples per day.
- Method: OD430nm
- Samples: Enzyme preparations, serum, plasma, tissue culture etc
ARGINASE (L-arginine ureohydrolase EC 3.5.3.1) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in breast, kidney, testes, salivary glands, epidermis and erythrocytes. Arginase catalyzes the conversion of arginine to ornithine and urea, completing the last step in the urea cycle. Arginase activity is a key diagnostic indicator. Increased levels of arginase activity in blood have been associated with liver damage. Hyperargininemia due to arginase deficiency is an inherited autosomal recessive disease. Simple, direct and automation-ready procedures for measuring arginase activity in biological samples are highly desirable in Research and Drug Discovery. BioAssay Systems arginase assay kit provides a sensitive and convenient method for arginase activity determination. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the arginase reaction. The intensity of the color is directly proportional to the arginase activity in the sample.