For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
- Linear detection range 2 - 500 μM pyruvate for colorimetric assays and 0.2 - 50 μM for fluorimetric assays.
- Convenient room temperature, high-throughput assay.
- Method: OD570 nm, or FL530/585 nm.
- Samples: Biological. Procedure: 20 min.
Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. BioAssay Systems pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.