For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
- Detection range colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
- Can be readily adapted to high-throughput assays for drug screening.
- Method: OD570 nm, or FL530/585 nm.
- Samples: Biological.
Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. BioAssay Systems method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.