Synonyms: Actin Antibody Sigma; Beta Actin Antibody - Monoclonal Anti-beta-Actin antibody produced in mouse; Beta Actin Antibody Sigma; Beta Actin Sigma Antibody; Sigma Actin Antibody; Sigma Beta Actin Antibody
MDL No.: MFCD00164531
Storage: -20C
UNSPSC Code: 12352203
General description: Recognizes an epitope located on the N-terminal end of the beta-isoform of actin. It specifically labels beta-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with beta-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin's solutions may be also used as fixatives. The antibody can be used as probes for beta-actin as an internal control in immunoblotting.
Application: Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Application: Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.
Application: Monoclonal anti-beta-actin antibody can be used for immunocytochemistry (10-40mug/mL using human or chicken fibroblasts), immunohistochemistry and microarray analysis.
Immunogen: slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Other Notes: To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
RIDADR: NONH for all modes of transport
WGK Germany: nwg