Synonyms: Monoclonal Anti-Human IgG (gamma-chain specific)
Storage: 2-8C
UNSPSC Code: 12352203
General description: Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
General description: Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000.
Application: Monoclonal Anti-Human IgG-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot. The product may also be used for dot blot (1:80,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
Application: Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).
Physical form: Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 3