Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Anti-His6-Peroxidase is used for the detection of His6-tagged proteins in:
• ELISA (enzyme-linked immunosorbent assay)
• Western blot
General description:
Anti-His6-Peroxidase is a monoclonal antibody to His6-tagged proteins, conjugated to horseradish peroxidase. Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the epitope-sequence location; however, it preferentially recognizes the C-terminal His6 epitope with high sensitivity.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function test: Western blot using extracts from cell line expressing a recombinant His6-tagged protein.
Preparation Note:
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
• ELISA: 100 mU/ml
• Western blot: 100mU/ml
Reconstitution:
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Reconstitution should be performed for at least 10 minutes.
Specificity:
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.