Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Analysis Note:
Preparation: After immunization with digoxigenin, sheep IgGs are purified by ion exchange chromatography. Specific IgGs are isolated by immunosorption. Fab fragments obtained after papain digestion are purified by gel filtration and lyophilized.
Features and Benefits:
Specific to digoxigenin and digoxin.
Application:
Anti-Digoxigenin, Fab fragments are used for the detection of digoxigenin-labeled compounds in:
• ELISA (enzyme-linked immunosorbent assay)
• Immunohistocytochemistry
• In situ hybridization
• Western blot
General description:
Anti-Digoxigenin, Fab fragments are Fab fragments from polyclonal anti-digoxigenin antibodies. Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: 2 to 4 μg/ml
• Immunohistocytochemistry: 0.5 to 1 μg/ml
• In situ hybridization: 0.2 to 0.4 μg/ml
• Western blot: 0.5 to 2 μg/ml
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
Reconstitution:
Add 1 ml PBS to a final concentration of 1 mg/ml. Centrifuge reconstituted antibody for 5 minutes at 10,000 rpm prior to each use, and pipet the necessary amount carefully from the surface.
Sequence:
The number of amino groups in the Fab fragments is not known, because polyclonal antibodies are not sequenced. But as a rule of thumb full length antibodies contain about 80 amino groups on average.
Specificity:
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.