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Jackson Immunoresearch

Cy3-AffiniPure F'2 Fragment Rabbit Anti-Goat IgG, F'2 Fragment Specific

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Cy3-AffiniPure F'2 Fragment Rabbit Anti-Goat IgG, F'2 Fragment Specific

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against the Fc portion of goat IgG or against non-immunoglobulin serum proteins. The antibody may cross-react with immunogloublins from other species.

Target: Goat

Host: Rabbit

Antibody Format: F(ab')2 Fragment

Specificity: IgG, F(ab')2 fragment specific

Conjugate: Cyanine Cy™3

Clonality: Polyclonal

BSA and dry milk may contain IgG which will be recognised by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titre.
This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Cy is a trademark of Cytiva Bio-Sciences Ltd. Jackson ImmunoResearch is licensed by Cytiva Bio-Sciences Ltd. to manufacture and sell conjugates of Cy2, Cy3, and Cy5 reactive dyes under US Patent Number 5,268,486, and other patents pending.

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6  
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative0.05% Sodium Azide 
Suggested Working Concentration or Dilution Range
Histo-/Cyto-chemistry1:100-1:800 
Flow Cytometry1:100-1:800 
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C839C16
Mfr. No.
305-166-006
Description
Cy3-AffiniPure F'2 Fragment Rabbit Anti-Goat IgG, F'2 Fragment Specific, 0.5 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C839C16 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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