Product is the lyophilized powder of goat antiserum to human serum and buffer salts.
The antiserum is prepared by hyperimmunizing goats with human serum. The resulting antiserum is delipidated and dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
USES: This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
(Note: IgG fractions are suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers and for attachment to solid supports for use as an immunoadsorbant.)
SPECIFICATIONS: The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized with an in-house control by immunoelectrophoresis. Each antiserum is tested for specificity at a minimum of 80 mg/ml using immunoelectrophoresis. It shows reactivity to human serum; cross-reactivity to other species may exist.
RECONSTITUTION: Reconstitute product with 2.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.