This antigen affinity gel is prepared by reaction of purified IgG to activated Sepharose® 4B gel. The washed gel is packaged as a ready-to-use column. The bed volume is 2 mL.
IgG affinity gel contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because this gel is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity. IgG affinity gel works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with this gel are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.
The chromatography gel can be used for affinity purification of antibodies to rabbit IgG or to remove these antibodies from an antiserum preparation. With appropriate elution buffers, the gel may be re-used.
IgG affinity gel comprise a simpler and faster alternative to traditional Protein A and Protein G methods for purification of rabbit IgG from serum and other sources.
Product is rabbit IgG (whole molecule) covalently coupled to Sepharose-4B, and packed in a polystyrene chromatography column.
Key Applications: Purification of rabbit antibodies
Application Areas: Immunoassays
Product Type: Reagents
Presentation: The chromatography gel is in 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v).
NOTES: Binding Capacity: 2.3 mg antibody/mL of gel
Specificity: The antigen reacts with anti-rabbit IgG and may cross-react to antibodies of other species. Each mL of gel has a minimum binding capacity of 2.0 mg of antibody.
Storage & Handling: Store product at 2-8°C. Addition of 0.05% sodium azide or other preservative to the equilibration buffer is recommended to inhibit microbial growth during storage. Do not freeze.